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Soil health is defined as the capacity of soil to function as a vital living ecosystem (Doran and Zeiss 2000), where a healthy soil is believed to be highly productive, optimally functional, and naturally able to recover from disturbances (Doran and Parkin, 1994; Kibblewhite et al. 2008). Numerous research groups have developed or are trying to develop soil health tests (Rinot et al. 2018), but none is universally accepted.

To maintain soil health, USDA-NRCS prescribes a core set of practices (USDA-NRCS 2018):

  1. Keep the soil covered as much as possible.
  2. Disturb the soil as little as possible.
  3. Keep plants growing throughout the year to feed the soil.
  4. Diversify as much as possible by using crop rotation and cover crops.

Many North Carolina farmers already use these core practices. For example, a corn, wheat, soybean rotation with conservation tillage provides all four soil health practices. The ability to use these practices depends on the specifics of the cropping system, but in general, crop rotation and conservation tillage have been important management practices implemented throughout North Carolina for several decades.

Many farmers want to know the usefulness of soil health testing. The two most currently used tests in the United States are the Haney Soil Health Test (Haney) and the Cornell Comprehensive Assessment of Soil Health (Cornell). The Haney test measures carbon dioxide respiration from microbes in the soil as a soil health indicator in addition to nutrient testing (Haney et al. 2006). The test is provided by commercial labs for around $55 per sample. The Cornell test was developed by the Cornell University Soil Health Testing Laboratory and includes multiple physical, chemical, and biological indicators in its evaluation (Moebius et al. 2007). It costs $110 per sample. The Haney and Cornell soil health tests do analyze more soil properties than traditional soil tests. However, there is no confirmation that these assessments of soil health indicators will lead to recommendations to improve soil health for different soils.

North Carolina has three physiographic regions—coastal plain, piedmont, and mountains—and long-term agronomic trials have been conducted in each of these regions. The long-term coastal plain trial is in Goldsboro (20 years), the piedmont trial is in Reidsville (35 years), and the mountain trial is in Mills River (25 years) (Figure 1). Each trial has a unique management history, but two factors in particular were compared generally at Goldsboro (coastal plain) and Mills River (mountain). Those factors were tillage (no-till vs conventional tillage) and management (conventional vs organic management). At Reidsville (piedmont), researchers studied tillage intensity ranging from no-till to moldboard plowing (Table 1).

Figure 1. Map of long-term tillage and cropping trials located a

Figure 1. Map of long-term tillage and cropping trials located across North Carolina. To the left is Mills River in the mountains. In the center is Reidsville in the piedmont. To the right is Goldsboro in the coastal plain.

Soil Health Results

The Haney test was only conducted at Reidsville and Mills River. There was substantial variation in soil health scores, ranging from 7 to 21, but any soil scoring 7 or more is considered to have “high” soil health on the Haney test (Table 1). Results from the Haney test determined that soil health was high for most of the treatments at the two locations, with the exception of spring moldboard at Reidsville, which scored “low” soil health. There was little differentiation between conservation and conventional tillage based on Haney ratings.

For the Cornell test, soils are rated on a scale from 0 to 100, with 0 indicating low soil health and 100 indicating high soil health. Overall soil health results from the Cornell tests did not demonstrate soil health differences for coastal plain or piedmont treatments (Table 1). All treatments at these two locations received low soil health scores regardless of tillage or management. At the Mills River location, no-till organic management had greater soil health than no-till conventional management, conventional tillage treatments, and the control. In fact, no-till organic management at Mills River was the only treatment to receive a moderate soil health score on the Cornell test because every other treatment received a low rating. An obvious difference in soil heath results exists between the Cornell and Haney tests.

Table 1. Haney and Cornell soil health scores and ratings by treatment at the long-term research trials. Different letters denote statistical differences. When there is no letter, there is no statistical difference. (From Roper et al. 2017).
Haney Score Haney Rating Cornell Score Cornell Rating
Goldsboro (coastal plain)
No-till, conventional 45 Low
Conventional tillage, conventional 38 Low
Conventional tillage, organic 1 43 Low
Conventional tillage, organic 2 46 Low
Reidsville (piedmont)
No-till 16 a High 43 Low
In-row subsoiling 46 Low
Disk, spring 46 Low
Chisel plow, fall 46 Low
Chisel plow, spring 46 Low
Chisel plow, disk fall 45 Low
Chisel plow, disk spring 8 b High 38 Low
Moldboard plow, fall 35 Low
Moldboard plow, spring 5 b Low 39 Low
Mills River (mountains)
No-till, conventional 12 High 44 b Low
No-till, organic 21 High 55 a Medium
Conventional tillage, conventional 7 High 48 ab Low
Conventional tillage, organic 19 High 49 ab Low
Control (no fertilizer or weed control) 8 High 44 b Low

Soil Organic Matter and Respiration Tests

Measuring soil organic matter content or respiration are the two most common tests for evaluating soil health. There are, however, a variety of ways to conduct these tests. Loss-on-ignition (LOI) is the most frequently used way to measure soil organic matter. Soil is dried, weighed, put in a very hot oven, and weighed again. The loss in weight in the oven is considered to be the organic matter that has “burned up.” Carbon content can also be measured directly with a C:N analyzer, which can detect carbon dioxide released from burned soil. Another long-used method, Walkley-Black, uses strong oxidizing chemicals to burn up the carbon in soil organic matter. It is often assumed that carbon makes up 58 percent of the weight of organic matter, so a factor of 1.72 is used to convert the measured carbon to organic matter. Each method has some limitations, and converting results from one method to another is problematic.

Soil respiration is determined by measuring carbon dioxide gas (emissions from a soil sample over a period of one to four days). Most studies indicate that the longer the test is run, the more stable the measurement, and that these values will change based on the length of the test. Table 2 provides a comparison of results for various organic matter and respiration tests conducted using soils from the three long-term trials.

Using organic matter content or CO2 respiration as soil health indicators can be problematic because the method of analysis used for a given test can produce very different soil organic matter and respiration measures for the same soil and treatment. For instance, soil organic matter for the spring disk treatment at Reidsville ranges from 0.9 to 2.53 percent depending on which technique and lab were used (Table 2). Additionally, some of these tests showed statistical differences in organic matter based on treatment, while others did not. For example, the Walkley-Black measurements for Goldsboro treatments suggest differences in soil organic matter among conventional tillage with conventional production, no-till with conventional production, and conventional tillage with organic production. None of the other tests—LOI or C:N Analyzer—demonstrates any statistical differences. At Reidsville and Mills River, organic matter was differentiated by treatment when measured in the NC State University lab but not in the Cornell lab. Since soil organic matter is such a critical part of determining soil health, it is essential that our methodologies be stable, include minimal error, and be reproducible across labs. Currently, that is not the case.

Table 2. Soil organic matter as measured at NC State and Cornell universities using loss-on-ignition (LOI) and by NC State University using a C:N analyzer and the Walkley-Black methodology. Carbon was converted to soil organic matter by multiplying the percentage of carbon by 1.72. The CO2 respiration was measured by the Cornell and Haney labs. Statistical differences are denoted by different letters. When there is no letter, there is no statistical difference. (From Roper et al. 2017; Roper et al. 2018).
Location and Treatment NC Organic Matter LOI Cornell Organic Matter LOI NC Organic Matter C:N Analyzer NC Organic Matter Walkley-Black Cornell Carbon Dioxide Respiration Haney Carbon Dioxide Respiration
% % % % mg g-1 mg L-1
Goldsboro (coastal plain)
No-till, conventional 1.71 1.83 1.41 2.24 a 0.35
Conventional tillage, conventional 1.71 1.43 1.53 1.82 b 0.29
Conventional tillage, organic1 1.78 1.43 1.45 2.26 a 0.40
Conventional tillage, organic 2 2.05 1.93 1.53 2.02 ab 0.38
Reidsville (piedmont)
No-till 2.18 bc 2.65 1.28 ab 2.02 0.41 ab 124 a
In-row subsoiling 2.92 a 2.89 1.61 a 2.38 0.42 a
Disk, spring 2.39 abc 2.53 0.90 b 2.43 0.44 a
Chisel plow, fall 2.35 abc 2.26 1.34 ab 2.11 0.40 ab
Chisel plow, spring 2.57 ab 2.64 1.38 ab 2.33 0.45 a
Chisel plow, disk fall 2.12 bc 2.42 1.21 ab 2.07 0.30 ab
Chisel plow, disk spring 2.31 abc 2.44 1.11 ab 2.06 0.37 ab 52 b
Moldboard plow, fall 2.20 b 2.33 0.90 b 1.85 0.19 b
Moldboard plow, spring 1.75 c 1.99 0.94 b 1.97 0.27 ab 34 b
Mills River (mountains)
No-till, conventional 2.81 ab 2.62 1.50 b 2.48 ab 0.35 80
No-till, organic 3.47 a 2.81 2.09 a 2.79 a 0.47 155
Conventional tillage, conventional 2.63 b 2.45 1.24 b 2.16 b 0.41 48
Conventional tillage, organic 2.99 ab 2.55 1.58 b 2.36 ab 0.35 158
Control 2.82 ab 2.52 1.50 b 2.55 ab 0.38 51

Crop Yields

Crop yield and growth are not considered in soil health tests. Nevertheless, due to the long-term nature of these agronomic trials, we were able to review yield differences based on treatments for many years.

Figure 2 shows changes in soybean yield along with associated rainfall at Reidsville over time. There were treatment differences in 1990, 2000, 2008, 2010, 2012, and 2014, but the pattern of these differences was not the same over years. Some years, no-till had the greatest yield, whereas other years in-row subsoiling had the greatest yield. Some years, moldboard plowing produced very low yields, but sometimes the yields were equivalent to no-till yields. Average soybean yield over time was about 38 bu ac-1 for all treatments combined, with no-till averaging 42 bu ac-1 and moldboard averaging 29 bu ac-1. Soybean yield from moldboard plowing was statistically less than no-till, chisel plow spring, and in-row subsoiling treatments when average yields over all years were considered.

Corn yields showed similar variation and trends to soybean yields at Reidsville (Figure 3). There were differences in treatments in 1987, 1988, 1989, 1993, 1997, 2007, and 2011. Overall yields were around 95 bu ac-1. The no-till treatment yielded more corn than chisel plow + disk, spring and moldboard plow, spring. Sweet corn yields at Mills River were greater for the conventionally managed treatments. Organic yields at Mills River were lower regardless of tillage, which was most likely due to weed pressure. Corn yields at Goldsboro averaged around 95 bu ac-1, and did not vary by treatment or year.

Figure 2. Soybean yield by treatment and year at Reidsville. NT

Figure 2. Soybean yield by treatment and year at Reidsville. NT = no-till; IRS = in-row subsoiling; CPS = chisel plow, spring; DS = disk, spring; CPDS = chisel plow + disk, spring; MPDS = moldboard plow, spring. Yield differences indicated with a *.

Figure 3. Corn yield by treatment and year at Reidsville. NT = n

Figure 3. Corn yield by treatment and year at Reidsville. NT = no-till; IRS = in-row subsoiling; CPS = chisel plow, spring; DS = disk, spring; CPDS = chisel plow + disk, spring; MPDS = moldboard plow, spring. Yield differences indicated with a *.


After comparing the Haney and Cornell soil health tests on three long-term trials, we saw very few differences in treatments for the measured properties. The Haney soil health ratings were mostly high, while the Cornell ratings were mostly low. We found no consistent relationship among soil health ratings, soil management, and crop yields. The one soil with better soil health as measured by the Cornell test was the organic management no-till treatment, yet sweet corn yield from that treatment was lower than the yields from all other treatments.

We recommend that farmers continue to manage for soil health and yield by:

  • using nutrient applications based on soil test results
  • rotating crops
  • maintaining as much soil cover as possible
  • minimizing tillage.

Due to the uncertainty surrounding the soil health measurements we observed, we have found that currently available tests may not provide a consistent means for evaluating progress on soil health management.

Acknowledgement and References

We thank the North Carolina Soybean Producers Association and the Environmental Defense Fund for funding the research discussed in this factsheet.

Doran, J. W. and M. R. Zeiss. 2000. “Soil Health and Sustainability: Managing the Biotic Component of Soil Quality.” Appl. Soil Ecol. 15(1): 3–11.

Haney, R. L., E. B. Haney, L. R. Hossner, and J. G. Arnold. 2006. “Development of a New Soil Extractant for Simultaneous Phosphorus, Ammonium, and Nitrate Analysis.” Commun. Soil Sci. Plant Anal. 37(11–12): 1511–1523.

Kibblewhite, M., K. Ritz, and M. Swift. 2008. “Soil Health in Agricultural Systems.” Philos. Trans. R. Soc. B Biol. Sci. 363(1492):685–701.

Moebius, B. N., H. M. van Es, R. R. Schindelbeck, O. J. Idowu, D. J. Clune, and J. E. Thies. 2007. “Evaluation of Laboratory-Measured Soil Properties as Indicators of Soil Physical Quality.” Soil Sci. 172(11): 895–912.

Rinot, O., G. J. Levy, Y. Steinberger, T. Svoray, G. Eshel. 2018. “Soil Health Assessment: A Critical Review of Current Methodologies and a Proposed New Approach.” Sci. Total Environ. 648:1484-1491.

Roper, W., D. Osmond, J. Heitman, M. Wagger, C. Reberg-Horton. 2017. “Soil Health Indicators Do Not Differentiate Agronomic Systems in North Carolina Soils.” Soil Sci. Soc. Amer. J. 81:828-843. doi: 10.2136/sssaj2016.12.0400.

Roper, W., D. Osmond, J. Heitman, and W. Robarge. 2018. “Comparing Four Methods to Measure Soil Organic Carbon in North Carolina Soils.” Soil Sci. Soc. Amer. J. (submitted)

U.S. Department of Agriculture-Natural Resources Conservation Service. 2018. Soil Health. Accessed 2018 September 15.


Department Extension Leader (Nutrient Mgt and Water Quality)
Crop & Soil Sciences
Graduate Student
Crop & Soil Sciences
Crop & Soil Sciences

Publication date: Nov. 19, 2018

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