NC State Extension Publications


Skip to Pathogen

Root-knot nematodes (Meloidogyne spp.) are microscopic roundworms that are widely distributed throughout North Carolina on many row and vegetable crops, and may cause varying degrees of damage to cotton. Specific root-knot nematode species that affect cotton include the southern root-knot (M. incognita), Javanese root-knot (M. javanica), and the more recently introduced guava root knot nematode (M. enterolobii). Of these, the southern root-knot (M. incognita) is the most wide-spread root-knot nematode in North Carolina. The guava root-knot nematode is an invasive species that has been reported in Florida, Louisiana, North Carolina, South Carolina, and Georgia. Across the globe, other official reports of the guava root-knot nematode include tropical to sub-tropical areas in Asia (China and Vietnam), South and West Africa (Malawi, Senegal, Ivory Coast and Burkina Faso), Central and South America (Mexico and Brazil), and the Caribbean islands, including Cuba, Puerto Rico, Tobago, Trinidad, Guadeloupe, and Martinique.

Host Range

Skip to Host Range

Root-knot nematodes (RKN) have a very extensive range of hosts, including several field crops grown in rotation with cotton in North Carolina such as tobacco, soybean, sweetpotato, and corn. Additionally, many vegetable crops, potatoes, and numerous weed species are hosts to root-knot nematodes. However, each specific species of root-knot nematode has an associated list of hosts, some of which overlap with other root-knot nematode species. These hosts are outlined in Table 1 below.

Life Cycle and Favorable Condition for Disease

Skip to Life Cycle and Favorable Condition for Disease

The life cycle of root-knot nematodes begins as an egg, goes through several successive juvenile stages, then reaches an adult stage. Eggs are produced in egg sacks at the root surface by female root-knot nematodes, which feed and survive fully inside the roots. The eggs survive in the soil until conditions are conducive for hatching. Infectious juvenile nematodes hatch from the eggs, move through the soil via water films, and invade nearby host roots. Once inside the host root, the juvenile root-knot nematodes will establish permanent feeding sites that will provide them sufficient nutrients for the rest of their life. The majority of the juveniles will become spherical-shaped adult females; a minor proportion will become adult males, which will leave the root and not feed. Adult female root-knot nematodes stimulate the root tissues at their feeding site to enlarge, producing the galls that are characteristic of infection by this nematode. Each female may lay hundreds of new eggs on the root surface, which then hatch in warm, moist soil to continue the life cycle.

The length of the life cycle depends on several factors, including soil temperature, soil moisture, and host plant suitability. under optimal conditions the life cycle takes approximately 25 days. Root-knot nematodes also require adequate soil moisture for egg hatching and movement through soil to host roots. Soil moisture conditions that are optimum for plant growth are also ideal for the development of root-knot nematodes.

Symptoms and Signs

Skip to Symptoms and Signs

Root-knot nematode infestations can often be identified in the field by the presence of galls, knots, or bumps on the root system of cotton plants. These root galls are localized swelling of root tissues initiated through feeding of the nematode inside the root (Figure 1).

Aboveground symptoms are frequently observed in “disease foci” or patches across the field, where the population density of nematodes is moderate to high. Plants in these patches may be chlorotic (yellow) and/or stunted. Other aboveground symptoms may include poor emergence, wilting, and early plant death. Foliar symptoms may indicate secondary infections caused by fungi or bacteria following the root damage caused by root-knot nematode feeding.

Root galling associated with guava root-knot nematode infected cotton

Figure 1. Meloidogyne enterolobii root galling symptoms on cotton.

Adrienne Gorny, NCSU


Skip to Identification

If a cotton crop is suspected to be impacted by root-knot nematodes, plants should be dug up, taking care to preserve the integrity of the root system, as possible. Roots should be washed gently in a bucket of water or under running water to remove soil. Visually inspect root, looking for the presence of root galls (Fig. 1), which indicate infection of the cotton plant by root-knot nematodes. Although the presence of root galls is sufficient to diagnose root-knot nematodes in cotton, root galling symptoms alone will not indicate the level of nematode pressure (nematode population counts) in the field, nor indicate what specific species of root-knot nematode is present. Diagnosis can be confirmed through assessment of soil and root samples for the presence of root-knot nematodes by a plant disease diagnostic laboratory or nematode assay lab. Soil samples for nematode assay services may be submitted to the North Carolina Department of Agriculture & Consumer Services (NCDA&CS), Agronomic Services, Nematode Assay Laboratory, 4300 Reedy Creek Road, Raleigh, NC 27607.

Nematode populations vary throughout a field, so multiple areas of the field should be sampled to provide an accurate evaluation of the entire field. Using a soil probe or hand trowel, collect 20 to 30 soil cores from a 5-acre block, at a depth of 6-8 inches, along either a grid-like or a zig-zag pattern across the field. Combine the soil cores in a clean plastic bucket and remove a smaller subsample from this larger sample to submit to the Nematode Assay Laboratory. Detailed sampling instructions and sample submission forms can be found at the NCDA&CS Nematode Assay Laboratory sampling guidelines.

The standard nematode soil assay cannot differentiate between specific root-knot nematode species, due to the highly similar appearance of these nematode species under the microscope. Root-knot nematode species identification can be obtained through a molecular assay at a diagnostic lab. This molecular diagnosis must be requested when submitting a sample and usually requires an additional fee. When requesting a molecular assay, it is helpful to include roots with distinct root galls at the time of sample submission as well as an accurate crop history, information about fertility, herbicides, and cultural practices to aid in diagnosis.


Skip to Management

Managing root-knot nematodes can be difficult due to their broad host range. Routine soil sampling for nematode analysis is important to better understand if root-knot nematodes are present in the field and if their population densities pose a risk to the cotton crop. Although soil samples for nematode analysis can be collected anytime in which the soil is not frozen and in good condition, sampling is best conducted in the fall, around harvest time, prior to Thanksgiving when nematode numbers are at their highest. It is valuable to know what the nematode pressures are in a given field to select the proper management tools.

Exclusion and Sanitation

Root-knot nematodes can be moved in infested soil via equipment, vehicles, tools, or shoes that have entered the field. Equipment from nematode-infested fields should be sanitized before moving to unaffected fields. Workers should also sanitize shoes after working in nematode-infested fields. Working in fields with known high populations of nematodes last will reduce time and effort needed for sanitation of equipment.

Resistant Varieties

Host resistance is one of the most cost-effective management tools for managing root-knot nematodes. There are cotton varieties available with resistance to southern root-knot nematode (M. incognita), which are listed in the Cotton Production Guide. To keep these RKN-resistant varieties functioning well and minimize the risk of developing populations of root-knot nematode that overcome the resistance, it is advised to rotate use of resistant and non-resistant varieties over several rotational cycles/years of cotton planting. No commercial cotton varieties with resistance to M. enterolobii are available at this time.

Crop Rotation

Crop rotation may decrease the nematode populations when a non-host crop is used as a rotation. Non-host and poor-host crops for root-knot nematodes, such as southern and guava root knot nematodes include sorghum, grasses, grain, alfalfa, peanuts, and resistant varieties of soybean and corn. Cotton is not economically affected by peanut root knot nematode (M. arenaria), and could be a good rotation option with peanuts (Table 1).

Table 1. List of host and non-host crops of root-knot nematodes reported in North Carolina
Root-knot nematode species Does this root-knot species infect cotton? Other Host Crops Non-host or poor-host crops
Southern root-knot nematode (Meloidogyne incognita) Yes (Races 3 and 4 of M. incognita infect cotton1) Soybean, tobacco, corn, small grains (wheat, oats, rye), tomato, watermelon, sweetpotato, potato, other vegetables Peanut, sesame, sunn hemp
Peanut root-knot nematode (Meloidogyne arenaria) No Peanut, tobacco, soybean, corn, small grains, sweetpotato, tomato, watermelon, sweetpotato Cotton, strawberry, sorghum-sudangrass
Javanese root-knot nematode (Meloidogyne javanica) Yes Soybean, tobacco, corn, small grains, sorghum, tomato, watermelon, potatoes Peanut, pepper, sunn hemp
Northern root-knot nematode (Meloidogyne hapla) No Soybean, tobacco, peanut, sweetpotato, tomato, potato, other vegetables Cotton, corn, watermelon, small grains, sorghum-sudangrass
Guava root-knot nematode (Meloidogyne enterolobii) Yes Soybean, tobacco, sweetpotato, tomato, potato, other vegetables Corn, peanut, small grains, sorghum-sudangrass

1Genetic host resistance is available in some cotton varieties to this species. Please see “Resistant Varieties” section.

Conventional Chemical Management

Chemical management options help to reduce populations of root-knot nematodes within the field and prevent infection of host plants. The products in Table 2 below have a label for use in cotton, as of 2023. For annually updated chemical management tools, consult the North Carolina Agricultural Chemical Manual.

Table 2. Chemical management options for suppression of root-knot nematodes in cotton. Table may not represent complete list of all products registered for suppression of nematodes in cotton in North Carolina, and inclusion of the products in the list does not endorse efficacy. Please refer to product label for application methods, regulations, and compliances.
Nematicide1,2 Amount/acre Precautions/Remarks
thidiocarb + imidacloprid (Aeris) 25.6 fl oz per 100 lbs seed seed treatment3
abamectin + thiomethoxam (Avicta Duo COT202) 0.15 mg a.i. abamectin per seed seed treatment3
Clothianidin plus Bacillus firmus I-1582 (Poncho/Votivo) 2.4 fl oz per 100,000 seed seed treatment3
fluopyram (Copeo) 1.13 to 1.69 fl oz per 100,000 seed seed treatment3
Aldicarb (AgLogic 15GG) 3.5 to 7 lbs Apply in-furrow and immediately cover with soil by mechanical means.
oxamyl (Vydate C-LV4) 17 fl oz Broadcast Vydate at 1st to 7th true leaf stage.
fluopyram (Velum) 6.84 fl oz Previously labeled as Velum Total; new formulation Velum does not contain imidacloprid, so an additional insecticide application may be necessary. Liquid applied in-furrow at planting.
1,3-dichloropopene (Telone II) 9 to 12 gallons (broadcast) Inject fumigant 1 to 2 weeks before planting 8 to 12 inches deep. See label for precautions and application requirements.
metam-sodium (Vapam HL; Metam CLR 42%) 30 gallons (broadcast) Inject fumigant 2 to 3 weeks before planting, 8 to 12 inches deep. See label for precautions and application requirements.

1See label for proper PPE, rates, and restrictions associated with each chemical. Chemical treatments listed are not exhaustive, and several seed treatment and in-furrow chemical options are available from different chemical companies.

2See restrictions for soil type and water proximity on label.

3Seed treatments are typically most effective under low to moderate root-knot nematode population counts.

4Vydate for root-knot and reniform nematodes only.

Useful Resources

Skip to Useful Resources


Assistant Professor and Extension Field Crop Pathology Specialist
Entomology & Plant Pathology
Extension Specialist (Nematology) & Assistant Professor
Entomology & Plant Pathology

Find more information at the following NC State Extension websites:

Publication date: Jan. 18, 2019
Revised: Aug. 7, 2023

N.C. Cooperative Extension prohibits discrimination and harassment regardless of age, color, disability, family and marital status, gender identity, national origin, political beliefs, race, religion, sex (including pregnancy), sexual orientation and veteran status.

NC Cooperative Extension prohíbe la discriminación por raza, color, nacionalidad, edad, sexo (incluyendo el embarazo), discapacidad, religión, orientación sexual, identidad de género, información genética, afiliación política, y estatus de veteran.

Recommendations for the use of agricultural chemicals are included in this publication as a convenience to the reader. The use of brand names and any mention or listing of commercial products or services in this publication does not imply endorsement by NC State University or N.C. A&T State University nor discrimination against similar products or services not mentioned. Individuals who use agricultural chemicals are responsible for ensuring that the intended use complies with current regulations and conforms to the product label. Be sure to obtain current information about usage regulations and examine a current product label before applying any chemical. For assistance, contact your local N.C. Cooperative Extension county center.

N.C. Cooperative Extension prohibits discrimination and harassment regardless of age, color, disability, family and marital status, gender identity, national origin, political beliefs, race, religion, sex (including pregnancy), sexual orientation and veteran status.