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Phoma macrostoma, a potential biocontrol agent for turfgrass weeds, was isolated from Cirsium arvense plants in Canada and is being tested in other regions of North America for control of broadleaf weeds in turf. This research was conducted to investigate the effects of varying temperature conditions on Phoma macrostoma control of seedling broadleaf weeds. Experiments were conducted in growth chambers to compare the efficacy of three doses of Phoma macrostoma on two species, Senecio vulgaris and Lamium amplexicaule grown in 4 temperature regimes – 15/20, 20/25, 25/30 and 30/35°C (dark / light period) temperatures. These data suggest that high temperatures common in the southeastern United States should not be an impediment to activity of Phoma macrostoma efficacy, and may actually improve the control of some broadleaf weed species.